latitude s (Gatan Inc)

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Gatan Inc latitude s
Latitude S, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/latitude+s/Latitude+S+Software/pm41861017-263-4-8
Average 99 stars, based on 118 article reviews

latitude s - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine Candidate.
Article Snippet: Prior to vitrification, R1.2/1.3 300-mesh gold grids (Quantifoil, Electron Microscopy Sciences) were glow-discharged at 20 mA for 130 s. Three μL of freshly prepared sample was added to the grid and incubated for 10 s before blotting for 7 s at 100% humidity, followed by rapid plunge freezing in liquid ethane using the FEI Vitrobot Mark IV plunger.

Article Title: Visualizing insecticide control of insect TRP channel function and assembly
Article Snippet: Movies of 40 frames each were collected using Latitude S (Gatan), with a nominal dose rate of 25 e – px −1 s −1 over 2.4 s exposure to give a total dose of ~60 e – Å −2 .

Mutagenesis:

Article Title: Development of a Thermostable and Broadly Neutralizing Pan-Sarbecovirus Vaccine Candidate
Article Snippet: Data acquisition was performed with the Talos Arctica (Thermo Scientific) at 200 kV, equipped with a K2 Summit Direct Electron Detector. .. Latitude S (Gatan) was used for automatic data collection at a nominal magnification of 54,000× with a pixel size of 0.92 Å at the specimen level, with a defocus range of −0.75 to −2.25 μm and a calibrated dose of 2–4 e – /Å 2 per frame for a total of 20 frames recorded over 8 s. A total of 3239 movies were acquired for the mutant S protein for image processing. ..

Software:

Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD
Article Snippet: For each data collection, the microscope was aligned using a cross-grating replica TEM grid; coma-free alignment and astigmatism corrections were done using the AutoCTF Sherpa functionality (Thermo Fisher Scientific). .. Data were collected using Latitude S [Digital Micrograph (Gatan) version 3.51] software running an image shift pattern at 0° stage tilt in counting mode with a nominal magnification of 81,000×/105,000× with a physical pixel size of 1.08/0.873 Å pixel and a defocus range of −0.8 to −2.5 μm. .. For grid preparation, UltrAufoil R1.2/1.3 Au 300 (Quantifoil) holey gold grids were glow-discharged (PELCO easiGlow) for 300 s, and 3 μl of purified samples of each three reconstituted complexes of DriD-ssDNA-RNAP-σ73-complexes with didA , CCNA_03891/CCNA_01149 , and bapE promoters were applied at room temperature at 90% humidity on separate grids.

Article Title: Transcription activation mechanism of a noncanonical DNA damage response pathway by the WYL-activator, DriD.
Article Snippet: For each data collection, the microscope was aligned using a crossgrating replica TEM grid; coma- free alignment and astigmatism corrections were done using the AutoCTF Sherpa functionality (Thermo Fisher Scientific). .. Data were collected using Latitude S [Digital Micrograph (Gatan) version 3.51] software running an image shift pattern at 0° stage tilt in counting mode with a nominal magnification of 81,000×/105,000× with a physical pixel size of 1.08/0.873 Å pixel and a defocus range of −0.8 to −2.5 μm. ..

Single Particle:

Article Title: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy.
Article Snippet: The data were collected on a Titan Krios transmission electron microscope (Thermo Fisher) operating at 300 kV equipped with a K3 direct electron detector (Gatan) in counting mode with a BioQuantum GIF energy filter (slit width of 20 eV) at a magnification of ×81,000 corresponding to a pixel size of 1.08 Å at the specimen level. .. 60-frame Videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of ~53-60 electrons per Å2 were collected using the Latitude-S (Gatan) single-particle data acquisition program. ..

Article Title: Antiparallel stacking of Csu pili drives Acinetobacter baumannii 3D biofilm assembly
Article Snippet: The data were collected on a Titan Krios transmission electron microscope (Thermo Fisher) operating at 300 kV equipped with a K3 direct electron detector (Gatan) in counting mode with a BioQuantum GIF energy filter (slit width of 20 eV) at a magnification of × 81,000 corresponding to a pixel size of 1.08 Å at the specimen level. .. 60-frame videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of 54.8 e-/Å 2 were collected using the Latitude-S (Gatan) single-particle data acquisition programme. ..

Article Title: Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy
Article Snippet: The data were collected on a Titan Krios transmission electron microscope (Thermo Fisher) operating at 300 kV equipped with a K3 direct electron detector (Gatan) in counting mode with a BioQuantum GIF energy filter (slit width of 20 eV) at a magnification of ×81,000 corresponding to a pixel size of 1.08 Å at the specimen level. .. 60-frame Videos with a dose rate of ~15 electrons per pixel per second and a total accumulated dose of ~53-60 electrons per Å 2 were collected using the Latitude-S (Gatan) single-particle data acquisition program. ..

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